DNMT3b SUMOylation Mediated MMP-2 Upregulation Contribute to Paclitaxel Induced Neuropathic Pain

Neurochemical Research - Paclitaxel is a common chemotherapeutic agent in cancer treatment, while it often causes chemotherapy-induced peripheral neuropathy (CIPN), which...     

Fecal Microbiota Transplantation Exerts a Protective Role in MPTP-Induced Parkinson’s Disease via the TLR4/PI3K/AKT/NF-κB Pathway Stimulated by α-Synuclein

Neurochemical Research - Gut microbiota is closely related to the Parkinson’s disease (PD) pathogenesis. Additionally, aggregation of α-synuclein (α-syn) is central to PD...     

Activation of Dopamine Signals in the Olfactory Tubercle Facilitates Emergence from Isoflurane Anesthesia in Mice

Neurochemical Research - Activation of dopamine (DA) neurons is essential for the transition from sleep to wakefulness and maintenance of awakening, and sufficient to accelerate the emergence from...     

琥珀蚕孵化酶基因AaHE的克隆及启动子活性分析

【目的】琥珀蚕Antheraea assama具有典型的野蚕特征,蚕卵孵化不齐,严重影响琥珀蚕的室内规模化饲养。本研究旨在探究对琥珀蚕卵孵化起关键作用的孵化酶(hatching enzyme)基因及其启动子序列特征,为进一步选择合适的抑制剂或促进剂调节琥珀蚕卵的孵化奠定基础。【方法】采用RACE技术克隆琥珀蚕孵化酶基因的cDNA全长序列,对基因序列进行生物信息学分析;采用qRT-PCR检测琥珀蚕孵化酶基因在琥珀蚕不同发育天数卵中及5龄第3和4天幼虫不同组织(丝腺、马氏管、头、中肠、脂肪体、表皮、血液、精巢和卵巢)中的表达情况;采用染色体步移克隆琥珀蚕孵化酶基因的启动子序列,构建昆虫细胞重组表达载体转染家蚕Bombyx mori BmN细胞,检测琥珀蚕孵化酶基因启动子活性。【结果】获得了琥珀蚕孵化酶基因AaHE(GenBank登录号： KT336227.1)全长cDNA序列,长993 bp,编码294个氨基酸,预测蛋白质分子质量为33.7 kD,理论等电点为5.17。AaHE氨基酸序列含有一个信号肽和一个ZnMc结构域,AaHE是一种含有HExxH锌结合位点的锌依赖性蛋白水解酶,该类酶既是肽酶,同时又是一种消化酶。AaHE在琥珀蚕孵化前的卵及5龄幼虫中肠中特异性高表达,分别与AaHE的肽酶和消化酶的属性相吻合。AaHE启动子核心区存在多个转录因子结合位点,这可能与转录因子参与调节AaHE的表达有关。启动子活性分析表明,AaHE启动子在家蚕BmN细胞中能够启动EGFP基因的表达,具有明显的启动子活性。【结论】AaHE是锌依赖性蛋白水解酶,其启动子核心区存在多个转录因子结合位点。本研究为选择合适的抑制剂或促进剂调节琥珀蚕卵的孵化率提供了参考。     

草地贪夜蛾海藻糖合成酶基因的克隆、时空表达谱及对温度胁迫的响应

【目的】本研究旨在通过克隆草地贪夜蛾Spodoptera frugiperda的海藻糖合成酶(trehalose-6-phosphate synthase)基因SfTPS,分析其在草地贪夜蛾不同发育阶段、不同组织中的表达水平及不同温度胁迫时5龄幼虫中的相对表达量,为进一步探究TPS在草地贪夜蛾生长发育及抗逆应激反应中的功能奠定基础。【方法】运用RT-PCR技术克隆草地贪夜蛾SfTPS的全长编码区,并进行生物信息学分析。运用RT-qPCR技术检测SfTPS在草地贪夜蛾不同发育阶段(卵、1-6龄幼虫、蛹和成虫)、5龄幼虫不同组织(体壁、中肠和脂肪体)中和经短期(2, 4和8 h)高(35℃)低温(10℃)胁迫后5龄幼虫中的表达变化。【结果】克隆获得2 571 bp的草地贪夜蛾TPS cDNA序列,命名为SfTPS(GenBank登录号： MT920672),全长开放阅读框(ORF)长2 481 bp,编码的826个氨基酸具有TPS和TPP两个保守结构域。同源比对和系统进化分析表明,昆虫TPS蛋白具有较高的保守性,SfTPS与斜纹夜蛾S. litura的TPS亲缘关系最近,序列一致性达到99.15%。SfTPS中α-螺旋、β-折叠和无规则卷曲占比分别为38.14%, 12.23%和48.55%;SfTPS的三级结构为同源二聚体。RT-qPCR结果表明,SfTPS在草地贪夜蛾卵期和1-5龄幼虫期低表达,在6龄幼虫期、蛹期和成虫期高表达,且草地贪夜蛾变态前后SfTPS的表达量变化较大。组织分布结果显示,SfTPS在5龄幼虫脂肪体中表达量最高。草地贪夜蛾5龄幼虫经2~8 h低温(10℃)和高温(35℃)胁迫后,SfTPS的相对表达量显著高于对照(25℃),分别为对照的4.43~9.34和2.50~6.03倍。【结论】SfTPS基因在草地贪夜蛾生长发育过程及抵御高低温度胁迫中可能具有重要作用。     

Plasticity of symbiont acquisition in new recruits of the massive coral Platygyra daedalea under ocean warming and acidification

Coral Reefs - Symbiosis establishment is a milestone in the life cycles of most broadcast-spawning corals; however, it remains largely unknown how initial symbiont infection is affected by ocean...     

The application of decellularized nucleus pulposus matrix/chitosan with transforming growth factor β3 for nucleus pulposus tissue engineering

Cytotechnology - Low back pain caused by intervertebral disc degeneration has become a global problem that seriously affects public health. The application of nucleus pulposus tissue engineering to...     

Restoration approach influences carbon exchange at in-situ oil sands exploration sites in east-central Alberta

Wetlands Ecology and Management - Oil sands exploration activities across the Alberta boreal peatlands requires tree clearing and results in sites being left compressed and with altered understory...